Forensic RNA-seq re-analysis of dataset GSE286438 to audit EGCG-mediated senescence reversal claims using PyDESeq2, PCA, correlation matrices, and biomarker validation.

Separating cell senescence from generic inflammation in IBD mucosa: SASP burden predicts biologic response (AUC 0.85/0.74); MR+coloc nominate CCL8 (risk) & CXCR2 (protective). Pure-Python, public-data pipeline.

It includes the figure depicting SASP heterogeneity in WI‑38 fibroblasts and per‑tissue differential expression results (senescent/stressed vs. control fibroblasts) derived from our ensemble-of-gene-sets (EGS) SASP scoring workflow.