This workflow performs differential gene expression and isoform splicing analysis on Nanopore long-read RNA-Seq data using minimap2, Salmon, and DESeq2.
Isoform-level detection, quantification, and differential expression analysis are integrated via FLAIR. Protein annotation, for example when ontological data are missing, is performed using Lambda.
Detailed usage instructions are available in the Snakemake Workflow Catalogue.