fluorescentIndicator.tpl.json

{
    "_type": "microscopy: fluorescentIndicator",
    "_extends": "/core/schemas/research/experimentalAcitivity.schema.tpl.json", 
    "required": [
        "name",
        "chemicalComposition",
        "fluorescentIndicatorTypes"
    ],
    "properties": {
        "name": {
            "type": "string",
            "_instruction": "Add canonical name of this fluorescent calcium indicator."
        }, 
        "chemicalComposition": {
            "_instruction": "Add the underlying molecular composition of the indicator.",
            "_linkedCategories": "chemicals:chemicalMixture" 
        },
        "fluorescentIndicatorTypes": {
            "_instruction": "Add classification of the indicator.",
            "_linkedCategories": "controlledTerms:?" 
        },
        "emissionPeak": {
            "_instruction": "Add peak emission wavelength of the indicator, expressed in nanometers.",
            "_linkedCategories": "core:QuantitativeValue" 
        },
        "onKinetics": {
            "_instruction": "Add rise time (on-kinetics) of fluorescence after calcium binding, typically in milliseconds.",
            "_linkedCategories": "core:QuantitativeValue"
        },
        "offKinetics": {
            "_instruction": "Add decay time (off-kinetics) of fluorescence after calcium unbinding, typically in milliseconds.",
            "_linkedCategories": "core:QuantitativeValue" 
        }, 
        "apparentDissociationConstant": {
            "_instruction": "Add apparent dissociation constant (binding affinity for Ca²⁺), typically in micromolar (µM).",
            "_linkedCategories": "core:QuantitativeValue" 
        }     
    }
}